IMDEA Nanociencia, Madrid, Spain. Duration: 2-3 years
We are looking to hire a Postdoctoral Research Assistant for an exciting project aimed to understand the dynamics of the human DNA replication in health and disease at the single-molecule level. Our lab (www.borjaibarralab.com) specializes in the single-molecule biophysics of DNA replication and employs state-of-the-art single molecule techniques. We have stablished national and international collaborations to drive cutting-edge research.
Role Overview:
The candidate will:
– Use optical trapping and fluorescence microscopy to study DNA replication on individual DNA molecules
– Use and develop data analysis routines to interpret experimental data.
– Acquire skills in protein purification and ensemble biochemistry.
– Contribute to building an outstanding interdisciplinary team together with other labs involved in the project, which are experts in the biochemistry (North Florida University), structural biology (CNIO, Madrid) and cell biology (CIB-CSIC, Madrid) of human mitochondria. Secondments will be encourage.
Project Description:
The selected candidate will investigate the dynamics of the human mitochondrial DNA replisome at the single-molecule level. In particular, the project will be focused on elucidating the real-time kinetics, stoichiometry, and exchange dynamics of the components of the mitochondrial replisome. We will also determine the effect of pathogenic variants of replisome factors on replisome operation. This will involve using novel biophysical instruments and collaborating with experts in molecular biology and biochemistry. Based on the relevance of mitochondrial DNA replication in health and disease and the novelty of the results, we expect high impact of our results.
Requirements:
– A Ph.D. (or near completion) in biophysics or fluorescence microscopy.
– Experience in the mentioned areas, with strong instrumentation and quantitative analysis skills.
– Motivation to develop a combined biophysics/biochemistry skillset.
– Independent, well-organized work style, with a team-oriented mindset.
– A strong publication record and excellent communication skills.
For more information and application contact borja.ibarra@imdea.org
Applications should include a motivation letter, CV, and contact information of three references. The closing date for applications is November 15th 2024.
